Journal: Life Science Alliance

Article Title: tRF-1:30-Gly-CCC-3 inhibits thyroid cancer via binding to PC and modulating metabolic reprogramming

doi: 10.26508/lsa.202302285

Figure Lengend Snippet: (A) The workflow of tRF and tiRNA sequencing and analysis. (B) The principal component analysis of tRF and tiRNA expression in four pairs of PTC tissues (T) and adjacent normal tissues (AT). (C) The correlation coefficient analysis of tRF and tiRNA expression in tumor tissues and ATs. (D) Venn diagram of tRFs and tiRNAs detected only in tumor tissues (red region), only in ATs (blue region), and in both tissues (purple region). (E) Distributions of different tRFs and tiRNAs in tumor tissues and ATs. (F) Heatmap of differentially expressed tRFs and tiRNAs in the two groups. (G) Volcano plot of down-regulated tRFs and tiRNAs (green dots) and up-regulated tRFs and tiRNAs (red dots) in tumor tissues compared with ATs. Fold change ≥ 1.5 and P -value < 0.05. (H) Differentially expressed tRFs and tiRNAs were separately calculated in PTC, and tRF-5c was the main down-regulated type (13/20). (I) Relative expression of tRF-30 in 30 pairs of tumor tissues and ATs was detected by qRT–PCR. (J) Relative expression of tRF-30 was detected by qRT–PCR in normal thyroid epithelial cells (Nthy-ori 3-1) and four PTC cell lines (BCPAP, TPC1, KTC1, and NPA87). Data were shown as mean ± SD. ( t test and Mann–Whitney U test, * P < 0.05, ** P < 0.01, and *** P < 0.001). Quantitative results were based on three independent experiments. Source data are available for this figure.

Article Snippet: The human PTC cell lines (BCPAP, TPC1, KTC1, NPA87) and the normal thyroid epithelial cells (Nthy-ori 3-1) were purchased from the iCell Bioscience Inc. All cell lines were identified by STR analysis and removed from mycoplasma contamination.

Techniques: Sequencing, Expressing, Quantitative RT-PCR, MANN-WHITNEY

Journal: Life Science Alliance

Article Title: tRF-1:30-Gly-CCC-3 inhibits thyroid cancer via binding to PC and modulating metabolic reprogramming

doi: 10.26508/lsa.202302285

Figure Lengend Snippet: (A, B) Overexpression and knockdown efficiencies of tRF-30 in BCPAP (A) and KTC1 (B) cells were verified by qRT–PCR. (C, D) tRF-30 suppressed the viability of BCPAP (C) and KTC1 (D) cells by CCK-8 assay. (E, F) tRF-30 inhibited the proliferation of BCPAP (E) and KTC1 (F) cells by colony formation assay. (G, H) tRF-30 repressed the invasion of BCPAP (G) and KTC1 (H) cells by transwell invasion assay. Scale bar = 50 μm. (I, J, K) Tumor growth in the oe-tRF-30 group (S1–S10) was suppressed compared with the oe-NC group (N1–N10). (L) tRF-30 down-regulated Ki-67 staining of PTC tumors in an in vivo mouse model by IHC assay. Scale bar = 50 μm. Data were triple replicated and shown as mean ± SD. ( t test and Mann–Whitney U test, * P < 0.05, ** P < 0.01, and *** P < 0.001). NC means negative control, sh means short hairpin RNA, and oe means overexpression. Source data are available for this figure.

Article Snippet: The human PTC cell lines (BCPAP, TPC1, KTC1, NPA87) and the normal thyroid epithelial cells (Nthy-ori 3-1) were purchased from the iCell Bioscience Inc. All cell lines were identified by STR analysis and removed from mycoplasma contamination.

Techniques: Over Expression, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Transwell Invasion Assay, Staining, In Vivo, MANN-WHITNEY, Negative Control, shRNA

Journal: Life Science Alliance

Article Title: tRF-1:30-Gly-CCC-3 inhibits thyroid cancer via binding to PC and modulating metabolic reprogramming

doi: 10.26508/lsa.202302285

Figure Lengend Snippet: (A) Box plot showed PC was up-regulated in thyroid cancer tissues according to the database. (B) Relative expression of PC mRNA was detected by qRT–PCR in Nthy-ori 3-1 and four PTC cell lines. (C) Relative expression of PC protein was detected in Nthy-ori 3-1 and four PTC cell lines by Western blot with its quantification result. GAPDH was used as a control. (D, E) Overexpression and knockdown efficiencies of PC in BCPAP and KTC1 cells were verified by qRT–PCR (D) and Western blot (E). (F, G) PC promoted the viability of BCPAP (F) and KTC1 (G) cells by CCK-8 assay. (H, I) PC accelerated the colony formation of BCPAP (H) and KTC1 (I) cells by clonogenic assay. (J, K) PC increased the invasion abilities of BCPAP (J) and KTC1 (K) cells by transwell invasion assay. Scale bar = 50 μm. Data were triple-replicated and shown as mean ± SD. ( t test and Mann–Whitney U test, * P < 0.05, ** P < 0.01, and *** P < 0.001). Protein expression was quantified by ImageJ analysis of Western blots. Full blots were provided in the Original Source Data file. Source data are available for this figure.

Article Snippet: The human PTC cell lines (BCPAP, TPC1, KTC1, NPA87) and the normal thyroid epithelial cells (Nthy-ori 3-1) were purchased from the iCell Bioscience Inc. All cell lines were identified by STR analysis and removed from mycoplasma contamination.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Over Expression, Knockdown, CCK-8 Assay, Clonogenic Assay, Transwell Invasion Assay, MANN-WHITNEY

Journal: Life Science Alliance

Article Title: tRF-1:30-Gly-CCC-3 inhibits thyroid cancer via binding to PC and modulating metabolic reprogramming

doi: 10.26508/lsa.202302285

Figure Lengend Snippet: (A, B) ELISA assay results showed that the effect of tRF-30 on citrate and pyruvate levels was partially attenuated by PC overexpression in BCPAP (A) and KTC1 (B) cells. (C) The schematic diagram of the mechanisms by which tRF-30 directly bound to PC, decreased its protein stability, and interfered with replenishment of the TCA cycle intermediates, thus affecting metabolic reprogramming and suppressing PTC progression. Data were triple-replicated and shown as mean ± SD. ( t test and Mann–Whitney U test, * P < 0.05, ** P < 0.01, and *** P < 0.001). NC means negative control, and oe means overexpression.

Article Snippet: The human PTC cell lines (BCPAP, TPC1, KTC1, NPA87) and the normal thyroid epithelial cells (Nthy-ori 3-1) were purchased from the iCell Bioscience Inc. All cell lines were identified by STR analysis and removed from mycoplasma contamination.

Techniques: Enzyme-linked Immunosorbent Assay, Over Expression, MANN-WHITNEY, Negative Control

Journal: Oncology Letters

Article Title: hsa_circ_0004846 enhances the malignant phenotype of papillary thyroid carcinoma cells via the miR‑142‑3p/PELI1 axis

doi: 10.3892/ol.2025.14949

Figure Lengend Snippet: hsa_circ_0004846 is upregulated in PTC. (A) Schematic diagram of the circularization of hsa_circ_0004846. (B) Divergent primers and convergent primers were used to amplify hsa_circ_0004846 in cDNA and gDNA. (C) RT-qPCR detection of hsa_circ_0004846 in 34 PTC tissues and adjacent non-tumor tissues. (D) Expression levels of hsa_circ_0004846 in normal human thyroid cells (Nthy-ori 3–1) and thyroid cancer cells (BCPAP, TPC-1 and IHH-4). Fluorescence images of hsa_circ_0004846-overexpressing or hsa_circ_0004846-depleted (E) TPC-1 and (F) IHH-4 PTC cells. The expression levels of hsa_circ_0004846 were detected by RT-qPCR. # P<0.05. circ, circular; Lv, lentivirus; NC, negative control; PTC, papillary thyroid carcinoma; RT-qPCR, reverse transcription-quantitative PCR; sh, short hairpin.

Article Snippet: The thyroid cancer cell line BCPAP, the PTC cell line TPC-1 and the normal human thyroid cell line Nthy-ori 3–1 were purchased from iCell Bioscience Inc., and were cultured in RPMI-1640 medium (Beijing Solarbio Science & Technology Co., Ltd.) supplemented with 10% fetal bovine serum (FBS; Zhejiang Tianhang Biotechnology Co., Ltd.).

Techniques: Quantitative RT-PCR, Expressing, Fluorescence, Negative Control, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Cancer Management and Research

Article Title: AP-2α expression in papillary thyroid carcinoma predicts tumor progression and poor prognosis

doi: 10.2147/CMAR.S167874

Figure Lengend Snippet: Western blotting and IHC assay of the expression of AP-2α ( A and B ). Western blot assay of AP-2α protein expression in Nthy-ori 3-1 and two PTC cell lines (BCPAP and TPC-1). β-actin was used as a loading control. Comparison of relative expression levels of AP-2α in the PTC cell lines with normal cell by Western blotting. ( C ) Normal tissue showed negative expression of AP-2α protein (upper and lower panel ×200). AP-2α IHC staining in PTC tissues is shown (upper and lower panel ×200) in weak (scoring intensity =1), moderate (scoring intensity =2), and strong (scoring intensity =3). ( D ) IHC grade scores between tumors and ANTs stained with monoclonal anti-AP-2α antibody. The staining intensity was scored with grades 0–3. The AP-2α expression levels of tcPTC are showed to be higher than fvPTC and cPTC. The statistical analysis was based on Mann–Whitney test. ** P <0.01. Abbreviations: ANTs, adjacent nontumor tissues; Ap-2α, activator protein 2α; cPTC, classical variant of PTC; fvPTC, follicular variant of PTC; IHC, immunohistochemical; PTC, papillary thyroid carcinoma; tcPTC, tall cell and diffuse sclerosing variant of PTC.

Article Snippet: Human PTC cell lines (BCPAP and TPC-1) were obtained from iCell Bioscience Inc. (Shanghai, China), and one normal human thyroid epithelial cell line (Nthy-ori3-1) was obtained from yipu biotechnology Co., Ltd (Wuhan, China).

Techniques: Western Blot, Expressing, Control, Comparison, Immunohistochemistry, Staining, MANN-WHITNEY, Variant Assay, Immunohistochemical staining

Journal: Cancer Management and Research

Article Title: AP-2α expression in papillary thyroid carcinoma predicts tumor progression and poor prognosis

doi: 10.2147/CMAR.S167874

Figure Lengend Snippet: AP-2α expression with focal adhesion as well as notch signaling. Notes: ( A and B ) The Gene Set Enrichment Analysis results showing the correlation of AP-2α- and PTC-related gene sets in MSigDB. Gene sets “notch signaling” and “focal adhesion” were enriched in AP-2α high expression phonotype. ( C–F ) Western blot analysis for the protein level of Notch1, MMP2, and AP-2α in PTC (BCAPAP and TPC-1) cell lines transfected with AP-2α siRNA for 24 hours. Abbreviations: AP-2α, activator protein 2α; MSigDB, the Molecular Signature Database; NC, negative control siRNA; PTC, papillary thyroid carcinoma.

Article Snippet: Human PTC cell lines (BCPAP and TPC-1) were obtained from iCell Bioscience Inc. (Shanghai, China), and one normal human thyroid epithelial cell line (Nthy-ori3-1) was obtained from yipu biotechnology Co., Ltd (Wuhan, China).

Techniques: Expressing, Western Blot, Transfection, Negative Control

Journal: Biomedical Optics Express

Article Title: Analysis of the key signaling pathway of baicalin that induces autophagy in papillary thyroid cancer via an optical resonator

doi: 10.1364/BOE.561007

Figure Lengend Snippet: (a) The molecule structure of baicalin. (b) The 24 h and 48 h cell viabilities of bcpap cells by CCK8 assay (c) The colony formation assay of bcpap cells after 10 days under baicalin treatment (d) The 24 h wound healing assay and 48 h migration and invasion transwell assay of bcpap cells under baicalin of 0, 80, 160µM(×20) (e) The 24 h apoptosis rates of bcpap cells under baicalin of 0, 80, 160µM with flow cytometry. The results were represented the means ± SD of triplicate independent experiments. ***P < 0.001, **P < 0.01, *P < 0.05.

Article Snippet: Cell viability was detected via Cell Counting Kit-8 (CCK-8) assay. bcpap cell lines were incubated with baicalin for 24 to 48 h, and then replaced with 100uL 10% CCK-8 reagent (TargetMOI, China) for 1 h at 37C.

Techniques: CCK-8 Assay, Colony Assay, Wound Healing Assay, Migration, Transwell Assay, Flow Cytometry

Journal: Biomedical Optics Express

Article Title: Analysis of the key signaling pathway of baicalin that induces autophagy in papillary thyroid cancer via an optical resonator

doi: 10.1364/BOE.561007

Figure Lengend Snippet: (a) Heatmap of autophagy and apoptosis markers between baicalin treated and control PTC cells. (b)The scores of autophagy, NGFR and thyroid cancer. (c) The molecular structure of NGFR and AMPK, and binding energy between baicalin by AutodockVina 1.2.2, the interaction value was -8.322 kcal/mol and -9.433 kcal/mol. (d) The COIP of AMPK and NGFR in bcpap cells. (e) The autophagosomes images by transmission electron microscope in baicalin treated and untreated PTC cells (Arrows indicated autophagosome) (f) The autophagosomes photoed by fluorescence microscope in baicalin treated PTC cells (×40) and autophagy flux with flow cytometry. **P < 0.01.

Article Snippet: Cell viability was detected via Cell Counting Kit-8 (CCK-8) assay. bcpap cell lines were incubated with baicalin for 24 to 48 h, and then replaced with 100uL 10% CCK-8 reagent (TargetMOI, China) for 1 h at 37C.

Techniques: Control, Binding Assay, Transmission Assay, Microscopy, Fluorescence, Flow Cytometry

Journal: Biomedical Optics Express

Article Title: Analysis of the key signaling pathway of baicalin that induces autophagy in papillary thyroid cancer via an optical resonator

doi: 10.1364/BOE.561007

Figure Lengend Snippet: (a) The 24 h and 48 h cell viabilities of bcpap and bc-shNGFR cells by CCK8 assay (b) The colony formation assay of bcpap and bc-shNGFR cells after 10 days under baicalin treatment (c) The 24 h apoptosis rates of bcpap cells under baicalin of 0, 160µM combined with dorsomorphin or rapamycin with flow cytometry. (d) The 24 h wound healing assay of bcpap cells and bc-shNGFR under baicalin of 0, 160µM combined with dorsomorphin or rapamycin. (e) 48 h migration and invasion transwell assay of bcpap cells and bc-shNGFR under baicalin of 0, 160µM combined with dorsomorphin or rapamycin (×20). (f) The autophagosomes photoed by fluorescence microscope in baicalin/dorsomorphin/rapamycin treated PTC cells (×40). and autophagy flux with flow cytometry. The results were represented the means ± SD of triplicate independent experiments. ***P < 0.001, **P < 0.01, *P < 0.05, ns had no significance.

Article Snippet: Cell viability was detected via Cell Counting Kit-8 (CCK-8) assay. bcpap cell lines were incubated with baicalin for 24 to 48 h, and then replaced with 100uL 10% CCK-8 reagent (TargetMOI, China) for 1 h at 37C.

Techniques: CCK-8 Assay, Colony Assay, Flow Cytometry, Wound Healing Assay, Migration, Transwell Assay, Fluorescence, Microscopy